| Lecture | Date | Key Words and Topics | Chapter |
|---|---|---|---|
| 1 | 1/21/26 | Course mechanics, overview of course subject matter | 1 |
| 2 | 1/23/26 | Light waves and rays, ray bending at interfaces, Snell’s law, lens function, ray diagrams | 2 |
| 3 | 1/26/26 | Image quality, geometrical optics versus wave optics, diffraction | 3 |
| 4 | 1/28/26 | Ray tracing, real and virtual images, minification and magnification, lens equations, Activity 1 | 3 |
| 5 | 1/30/26 | Simple microscope, ray diagram, eyepiece, objective, critical and Kohler illumination | 3 |
| 6 | 2/2/26 | Resolution, diffraction, Rayleigh theory, Activity 2, numerical aperture | 4 |
| 7 | 2/4/26 | Abbe theory (start) | 4 |
| 8 | 2/6/26 | Abbe theory (finish), Activity 3, introduction to contrast | 4,5 |
| 9 | 2/9/26 | Amplitude and phase specimens, brightfield microscopy, tricks to enhance contrast, Kohler ray trace, conjugate planes, Activity 4, darkfield microscopy (start) | 5 |
| 10 | 2/11/26 | Darkfield microscopy (finish), phase contrast microscopy (start) | 5 |
| 11 | 2/13/26 | Phase contrast microscopy (continued) | 5 |
| 12 | 2/16/26 | Phase contrast microscopy (finish), Activity 5, introduction to fluorescence, overview of fluorescence microscopy, Jablonski diagram | 5,6 |
| 13 | 2/18/26 | Fluorescence excitation and emission, photons, quantification of light energy | 6 |
| 14 | 2/20/26 | Excitation, emission, spectra, fluorescence microscope (light source, emission filter, dichromatic mirror, stage, emission filter, detector), Activity 6, multi-color imaging | 6 |
| 15 | 2/23/26 | Dichromatic mirrors for multi-color imaging, 3D imaging, optical sectioning, blur problem, introduction to confocal microscopy | 6 |
| 16 | 2/25/26 | Blur problem, point spread function (PSF), laser scanning confocal microscopy (LSCM), pinhole size | 6 |
| 17 | 2/27/26 | Spinning disk confocal microscopy (SDCM), Nipkow disk, camera, comparison of LSCM and SDCM | 6 |
| 18 | 3/2/26 | Widefield fluorescence microscopy, deconvolution, polarization microscopy, Activity 7, crossed polarizers | 5,6 |
| 19 | 3/4/26 | Specialized fluorescence techniques (overview), two-photon microscopy (start) | 7 |
| 20 | 3/6/26 | Two-photon microscopy (finish), total internal reflection fluorescence microscopy (TIRFM) (start) | 7 |
| 21 | 3/9/26 | Total internal reflection fluorescence microscopy (finish), super-resolution fluorescence microscopy (overview), structured illumination microscopy (SIM) (start), moiré patterns | 7,8 |
| 22 | 3/11/26 | Structured illumination microscopy (finish), beats, photoactivatable localization microscopy (PALM) (start) | 8 |
| 23 | 3/13/26 | Photoactivatable localization microscopy (finish), stimulated emission depletion (STED) microscopy | 8 |
| 24 | 3/16/26 | ||
| 25 | 3/18/26 | ||
| 26 | 3/20/26 | ||
| 27 | 3/30/26 | ||
| 28 | 4/1/26 | ||
| 29 | 4/3/26 | ||
| 30 | 4/6/26 | ||
| 31 | 4/8/26 | ||
| 32 | 4/13/26 | ||
| 33 | 4/15/26 | ||
| 34 | 4/17/26 | ||
| 35 | 4/20/26 | ||
| 36 | 4/22/26 | ||
| 37 | 4/24/26 | ||
| 38 | 4/27/26 | ||
| 39 | 4/29/26 |